GABARAP anticorps (Intracellular, N-Term)

N° du produit ABIN7885007

Cet anticorps anti-GABARAP Polyclonal Lapin (ABIN7885007) détecte spécifiquement GABARAP dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour GABARAP anticorps (Intracellular, N-Term) (ABIN7885007)

Antigène: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GABARAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Classe de qualité: Carrier-free

Détail du produit anti-GABARAP anticorps

Épitope: AA 2-15, Intracellular, N-Term

Fonction: A Rabbit Polyclonal Antibody to GABA Receptor Associated Protein

Homologie: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)KFVYKEEHPFEKRR, corresponding to amino acid residues 2-15 of rat GABARAP

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:800

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235469)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur GABARAP

Antigène: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Autre désignation: GABA receptor associated protein

Sujet:

Synonyms: GABA receptor associated protein, GABARAPL

Description: GABA(A) receptors are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABA associated protein (GABARAP) family interacts specifically with the γ1, γ2S and γ2L subunits of the GABA(A) receptor. GABARAP is expressed in a variety of tissues including heart, brain, placenta, liver and others. The genetic sequence encoding GABARAP is similar to microtubule-associated proteins 1A and 1B1.GABARAP has an asymmetric structure with a basic N-terminal region and an acidic C terminus. GABARAP has a compact fold consisting of four or five-stranded β-sheets with two α-helices on each side. The outer strands of the β-sheets are aligned anti-parallel to the inner strands and helices α3 and α4 are located on one side of the sheet. The presence of α1 and α2 is a unique feature of GABARAP and forms a hydrophilic patch suitable for protein-protein interface. GABARAP exists in a monomeric conformation at low protein concentration and a head-to-tail oligomeric conformation in high salt conditions.GABARAP is involved in intracellular trafficking and is an integral part of the intra-Golgi transport machinery. Thus it is detected in cellular compartments such as the Golgi apparatus and endoplasmic reticulum. GABARAP affects GABA(A) receptors' cell surface expression by increasing their intracellular trafficking. Disruption of cellular microtubules prevents the clustering of both GABARAP and GABA(A) receptors. GABARAP also interacts with other receptors such as the glycine receptor at the postsynaptic site2.

ID gène: 58974

UniProt: P60517

Pathways: Autophagy