Gephyrin anticorps (Intracellular)

N° du produit ABIN7885038

Cet anticorps Lapin Polyclonal détecte spécifiquement Gephyrin dans WB, IHC et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Gephyrin anticorps (Intracellular) (ABIN7885038)

Antigène: Gephyrin (GPHN)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Gephyrin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Classe de qualité: Carrier-free

Détail du produit anti-Gephyrin anticorps

Épitope: AA 183-195, Intracellular

Fonction: A Rabbit Polyclonal Antibody to Gephyrin

Homologie: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235588)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur Gephyrin

Antigène: Gephyrin (GPHN)

Autre désignation: GPHN

Sujet:

Synonyms: GPHN, Putative glycine receptor-tubulin linker protein, MPT adenylyltransferase, MPT Mo-transferase

Description: Gephyrin is a highly conserved, intracellular scaffolding protein responsible for the post-synaptic anchoring and assembly of the inhibitory glycine and γ-aminobutyric acid type A receptors (GlyR and GABAAR), it essentially anchors the receptors, by auto-oligomerization, to the cytoskeleton1,2.GABAergic transmission is important for the mediation of neuronal excitability and has a crucial role in neurodevelopment, throughout the central and peripheral nervous systems (especially via GABAAR). Glycinergic neurons on the other hand control motor functions and sensory signaling as well as take part in pain transmission in the spine's dorsal horn. Importantly, gephyrin has a 1:1 stoichiometry with GlyR3,4.Up-to-date atomic force microscopy data of gephyrin has shown that it forms trimers with multiple conformations. It is a modular construct with three domains: an N-terminal G-, a central C- and a C-terminal E-domain. Recent work suggests also that gephyrin regulates neuronal differentiation by anchoring small cytoskeletal-related signaling molecules at unique subcellular locations2,5,6.

ID gène: 64845

UniProt: Q03555

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development