Granulin anticorps (Secreted)

N° du produit ABIN7885072

L’anticorps anti-Granulin Polyclonal Lapin est utilisé pour la détection de Granulin dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IHC.

Aperçu rapide pour Granulin anticorps (Secreted) (ABIN7885072)

Antigène: Granulin (GRN)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Granulin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Classe de qualité: Carrier-free

Détail du produit anti-Granulin anticorps

Épitope: AA 252-267, Secreted

Fonction: A Rabbit Polyclonal antibody to Progranulin

Homologie: Mouse - 15 out of 16 amino acid identical, Human- 14 out of 16 amino acid identical, Rat- 13 out of 16 amino acid identical

Purification: Affinity purified on immobilized antigen.

Immunogène: CDLIQSK(S)LSKNYTTD, corresponding to amino acid residues 252 - 267 of mouse GRN

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur Granulin

Antigène: Granulin (GRN)

Autre désignation: Granulin Precursor

Sujet:

Synonyms: Granulin Precursor, PGRN, Epithelin Precursor, Glycoprotein 88, GP88, PC Cell-Derived Growth Factor, PCDGF, GRN

Description: The Progranulin (PGRN) protein is encoded by the GRN gene composed of 13 exons, although exon 1 is non-coding. PGRN is a secreted glycoprotein with growth factor like properties, consisting in 7.5 tandem repeats of a 12-cysteine module called granulin or epithelin domain separated by linker regions. The 12-cysteine motif forms six disulfide bridges with the peptide backbone adopting a unique three-dimensional structure of a parallel stack of beta hairpin1,2.PGRN is synthesized as a 68.5 kDa protein that is N-glycosylated to become a mature secreted 88 kDa protein3. Secreted PGRN is cleaved into 6 kDa granulin peptides, each composed of cysteine repeat motifs. Cleavage of PGRN occurs at the intra-linker spacer regions by several proteases including elastase4, proteinase5, matrix metalloproteinase-146 and ADAM metallopeptidase with thrombospondin type 1 motif7. PGRN cleavage is inhibited by secretory leukocyte protease inhibitor (SLPI)4 and high-density lipoprotein (HDL)/Apolipoprotein A-I complex8.PGRN is expressed in several tissues showing a prominent expression in epithelial, immune and, neuronal cells9. In particular, a strong expression of PGRN mRNA was found in highly proliferative epithelia including those of the intestinal crypt, skin, and reproductive tracts9. In contrast, PGRN shows low expression in less proliferative and highly differentiated cells such as those of alveolar lung and renal tube epithelia9.PGRN shows high expression in peripheral immune system tissues such as spleen and lymph nodes9 and is also highly expressed in the central nervous system (CNS) with expression in both neurons and neuroinflammatory cells. PGRN has been detected in neurons of the neocortex, hippocampus, arcuate nucleus and ventromedial hypothalamus, Purkinje cells of the cerebellum and in motor neurons9. In the CNS, PGRN can function as neurotrophic growth factor increasing cell viability10 and neurite outgrowth [11]. PGRN knockdown in rat hippocampal neurons results in decreased neuronal arborization and length and synaptic densities12 showing its role in neuronal morphology and establishing interneuron connections.Several proteins have been identified as receptors for GPRN, including Sortilin, which is considered the major regulator of GPRN in the brain, as well as, EphA2, LRP1, Notch and more13.Loss of function mutations in the GRN gene, cause a range of neurodegenerative disorders including frontotemporal dementia (FTD), amyotrophic lateral sclerosis (ALS), Alzheimer's and Parkinson's disease 13.

ID gène: 14824

UniProt: P28798