NUCB2 anticorps (Intracellular)

N° du produit ABIN7885204

L’anticorps anti-NUCB2 Polyclonal Lapin est utilisé pour la détection de NUCB2 dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB et IHC.

Aperçu rapide pour NUCB2 anticorps (Intracellular) (ABIN7885204)

Antigène: NUCB2 (Nucleobindin 2 (NUCB2))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NUCB2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Classe de qualité: Carrier-free

Détail du produit anti-NUCB2 anticorps

Épitope: AA 30-44, Intracellular

Fonction: A Rabbit Polyclonal Antibody to Nucleobindin-2/Nesfatin-1

Homologie: human -11,Mouse -13,15 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)DKTKVHNVEPVESAR, corresponding to amino acid residues 30-44 of rat Nucleobindin-2/Nesfatin-1

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:1200

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7236159)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur NUCB2

Antigène: NUCB2 (Nucleobindin 2 (NUCB2))

Autre désignation: NUCB2

Sujet:

Synonyms: NUCB2, NEFA, Prepronesfatin, DNA-binding protein NEFA, Gastric cancer antigen Zg4, HEL-S-109

Description: Nucleobindin contains multiple functional domains including a signal peptide on the N-terminal side, a leucine/isoleucine rich region, a DNA binding domain and a putative nuclear targeting signal. The second half of the protein contains two Ca2+-EF-hand motifs and a leucine zipper motif in the C-terminal region. To date, two nucleobindins have been identified, namely nucleobindin 1 and nucleobindin 2.Nucleobindin 2 (NUCB2) can be found on the plasma membrane and in the cytoplasm. Putative post-translational processing of NUCB2 by the enzyme pro-hormone convertase (PC)-1/3 results in nesfatin-1, nesfatin-2 and nesfatin-3. So far, a biological activity has only been demonstrated for nesfatin-1 and its fragment, nesfatin-124-531.The gene Nesfatin/NUCB2 encoding the protein is expressed in the appetite control hypothalamic nuclei such as the paraventricular nucleus (PVN), the supraoptic nucleus, the lateral hypothalamic area and the zona increta in rats. Nefstain-1 immunoreactivity is also detected in the brain stem nuclei such as the nucleus of the solitary tract and the dorsal nucleus of the vagus.Nesfatin-1 has a substantial anorexigenic effect through the selective reduction of nesfatin/NUCB mRNA expression in the PVN of rats under starved conditions. Refeeding of rats was found to restore Nesfatin-1 reduction. A similar effect was not observed in other hypothalamic nuclei2. In humans, Nesfatin-1 levels in the saliva and serum of epilepsy patients are reduced with the use of anti-epileptic medication, but are still higher than the amounts in normal subjects. In addition, nesfatin-1 levels are decreased in human subjects with type-2 diabetes and it has been hypothesized that the observed decrement might be associated with diabetic hyperphagia3.

ID gène: 59295

UniProt: Q9JI85