BRD7 anticorps (C-Term)

N° du produit ABIN7938068

L’anticorps anti-BRD7 Polyclonal Lapin est utilisé pour la détection de BRD7 dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour BRD7 anticorps (C-Term) (ABIN7938068)

Antigène: BRD7 (Bromodomain Containing 7 (BRD7))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRD7 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-BRD7 anticorps

Épitope: C-Term

Fonction: BRD7 antibody (pAb)

Purification: Affinity Purified

Immunogène: This BRD7 antibody was raised against a peptide within the C-terminal region of human BRD7.

Isotype: IgG

Information d'application

Indications d'application: WB*: 1:500 - 1:2,000 dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in 70 mM Tris, 105 mM NaCl, 0.07 mM EDTA, 31 mM glycine, 0.035 % sodium azide, 30 % glycerol, 0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur BRD7

Antigène: BRD7 (Bromodomain Containing 7 (BRD7))

Autre désignation: BRD7

Sujet:

Background_Plain: BRD7 (Bromodomain-containing protein 7, aka CELTIX1) is a chromatin-associated protein component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 inhibit4ed cancer cell growth and metastasis and promotes apoptosis in vitro and in vivo via downregulating AKT pathway. BRD7 may also regulate signaling pathways like ras-raf-MEK-ERK and RB/E2F. Previous studies have also demonstrated that BRD7 was downregulated in huma breast cancer, and the downregulation is associated with tumor progression.

Aliases: Bromodomain containing 7, CELTIX1, BP75, SMARCI1

Poids moléculaire: 75 kDa

NCBI Accession: NM_013263