BRD9 anticorps (C-Term)

N° du produit ABIN7938072

Cet anticorps anti-BRD9 Polyclonal Lapin (ABIN7938072) détecte spécifiquement BRD9 dans WB et IP. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour BRD9 anticorps (C-Term) (ABIN7938072)

Antigène: BRD9 (Bromodomain Containing 9 (BRD9))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRD9 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-BRD9 anticorps

Épitope: C-Term

Fonction: BRD9 antibody (pAb)

Purification: Affinity Purified

Immunogène: This antibody was raised against a peptide within the C-terminal region of human BRD9 (bromodomain containing 9).

Isotype: IgG

Information d'application

Indications d'application: IP: 5 µL per IP WB*: 1:250 - 1:1,000 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal IP results, the addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur BRD9

Antigène: BRD9 (Bromodomain Containing 9 (BRD9))

Autre désignation: BRD9

Sujet:

Background_Plain: BRD9 (bromodomain containing 9) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6) that function in the regulation of transcriptional activation and chromatin remodeling. There are five isoforms of BRD9 that are produced by alternative splicing.

Aliases: PRO9856, LAVS3040

Poids moléculaire: 85 kDa

NCBI Accession: NP_076413