Histone H2A anticorps (H2AK119ub)

N° du produit ABIN7938165

L’anticorps anti-Histone H2A Polyclonal Lapin est utilisé pour la détection de Histone H2A dans des échantillons de Humain. Il a été validé pour WB et DB.

Aperçu rapide pour Histone H2A anticorps (H2AK119ub) (ABIN7938165)

Antigène: Histone H2A

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H2A est non-conjugé

Application: Western Blotting (WB), Dot Blot (DB)

Détail du produit anti-Histone H2A anticorps

Épitope: H2AK119ub

Fonction: Histone H2AK119ub antibody (pAb)

Purification: Affinity Purified

Immunogène: This antibody was raised against a branched peptide corresponding to the ubiquitin conjugation site at Lys119 of human Histone H2A.

Isotype: IgG

Information d'application

Indications d'application: DB: 1:1000 WB*: 1:1000 - 1:2000 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal results, we recommend the addition of 0.05 % Tween-20 to all blocking solutions to reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Buffer: 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol, and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur Histone H2A

Antigène: Histone H2A

Sujet: In humans, attachment of ubiquitin to Histone protein 2A (H2A) on lysine residue 119 (H2AK119Ub) is one of the most prevalent histone marks, and is estimated to occur on 5-15 % of histones genome-wide4. In general, H2AK119Ub is characteristic of repressed developmental genes, and is also tightly linked to levels of other histone marks such as lysine methylation and acetylation. H2AK119Ub is attached to histones by the Really Interesting New Gene (RING) ubiquitin E3-ligase Polycomb Repressive Complex 1 (PRC1), and removed by the Polycomb Repressive-Deubiquitinase (PR-DUB) complex, which plays a vital role in regulating H2AK119Ub histone marks, and mutations in human PR-DUB genes lead to a spectrum of malignancies.

Poids moléculaire: 23 kDa

NCBI Accession: NP_003508