Histone H2A anticorps (AA 122-129)

N° du produit ABIN7938167

Cet anticorps Lapin Polyclonal détecte spécifiquement Histone H2A dans WB et DB. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour Histone H2A anticorps (AA 122-129) (ABIN7938167)

Antigène: Histone H2A

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H2A est non-conjugé

Application: Western Blotting (WB), Dot Blot (DB)

Détail du produit anti-Histone H2A anticorps

Épitope: AA 122-129

Fonction: Histone H2A.J antibody (pAb)

Purification: Affinity Purified

Immunogène: This antibody was raised against a peptide comprising amino acids 122-129 of human Histone H2A.J.

Isotype: IgG

Information d'application

Indications d'application: WB*: 1:500 - 1:2500 dilution DB: 1:1000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur Histone H2A

Antigène: Histone H2A

Autre désignation: Histone H2A.

Sujet: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, and is responsible for establishing higher-order chromatin structure. Histone H2A.J is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. Histone H2A.J, which is found only in mammals, is shown to be associated with senescence, accumulating in senescent fibroblasts and promoting expression of inflammatory genes. Therefore, H2A.J may contribute to chronic inflammation and age-related diseases.

NCBI Accession: NP_808760