Histone H2A anticorps (C-Term)

N° du produit ABIN7938168

Cet anticorps anti-Histone H2A Monoclonal Souris (Clone 10E9-D1) (ABIN7938168) détecte spécifiquement Histone H2A dans WB et ChIP. L’anticorps est réactif avec des échantillons de Drosophila melanogaster.

Aperçu rapide pour Histone H2A anticorps (C-Term) (ABIN7938168)

Antigène: Histone H2A

Reactivité: Drosophila melanogaster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H2A est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Clone: 10E9-D1

Détail du produit anti-Histone H2A anticorps

Épitope: C-Term

Fonction: Histone H2Av antibody (mAb) (Clone 10E9.D1), sample

Purification: Protein A Chromatography

Immunogène: This antibody was raised against a peptide in the C-terminus of the Drosophila melanogaster Histone variant H2Av.

Isotype: IgG2a

Information d'application

Indications d'application: ChIP: 5 μg per ChIP WB: 0.5 - 2 μg/mL dilution

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur Histone H2A

Antigène: Histone H2A

Sujet: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle.In Drosophila, the H2A variant corresponding to H2AZ is H2Av. H2Av is an essential protein in Drosophila and has been implicated in both activation and repression of transcription. H2Av is localized to centromeric heterochromatin in Drosophila and flies lacking H2Av have reduced levels of heterochromatin components at the centromeres. However, H2Av nucleosome distribution throughout the rest of the Drosophila genome correlates with genes that have an open and uniform chromatin architecture at promoter regions.

Poids moléculaire: 15 kDa

NCBI Accession: NP_524519