Recombinant Histone H2A anticorps (H2A.XS139ph)

N° du produit ABIN7938169

Cet anticorps Lapin Monoclonal détecte spécifiquement Histone H2A dans WB et ICC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant Histone H2A anticorps (H2A.XS139ph) (ABIN7938169)

Antigène: Histone H2A

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H2A est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: RM224

Détail du produit anti-Histone H2A anticorps

Épitope: H2A.XS139ph

Fonction: Histone H2A.XS139ph antibody (rAb) (H2AX) (Clone RM224)

Purification: Protein A Chromatography

Immunogène: A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139).

Isotype: IgG

Information d'application

Indications d'application: WB: 0.5 - 2 μg/mL ICC: 0.5 - 2 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: PBS with 50 % glycerol, 1 % BSA, and 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur Histone H2A

Antigène: Histone H2A

Autre désignation: Histone H2A.

Sujet: Histone H2AX phospho Ser139 (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation. RM224 reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

NCBI Accession: NP_002096