METTL3 anticorps (N-Term)

N° du produit ABIN7938336

L’anticorps anti-METTL3 Monoclonal Souris est utilisé pour la détection de METTL3 dans des échantillons de Humain. Il a été validé pour WB et IP.

Aperçu rapide pour METTL3 anticorps (N-Term) (ABIN7938336)

Antigène: METTL3 (Methyltransferase Like 3 (METTL3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp METTL3 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 29C8

Détail du produit anti-METTL3 anticorps

Épitope: N-Term

Fonction: METTL3 antibody (mAb) (Clone 29C8)

Purification: Unpurified

Immunogène: This antibody was raised against a peptide within the N-terminal region of human METTL3 (N6-adenosine-methyltransferase).

Isotype: IgG2a

Information d'application

Indications d'application: WB: 1:500 - 1:1000 dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91333.

Restrictions: For Research Use only

Stockage

Buffer: This antibody is provided as a hybridoma culture supernatant with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur METTL3

Antigène: METTL3 (Methyltransferase Like 3 (METTL3))

Autre désignation: METTL3

Sujet:

Background_Plain: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also occurs in other RNA molecules, such as primary miRNA (pri-miRNAs). METTL3 (methyltransferase-like 3, also known as IME4, M6A, MT-A70) forms a stable N6-methyltransferase heterodimer complex with METTL14, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition. METTL3 promotes translation by interacting with the translation initiation machinery in the cytoplasm. Its overexpression in a number of cancer cells suggests that it may participate to cancer cell proliferation by promoting mRNA translation.

Aliases: Methyltransferase Like 3, MRNA (2-O-Methyladenosine-N(6)-)-Methyltransferase

Poids moléculaire: 72 kDa

NCBI Accession: NP_062826