PCIF1 anticorps (C-Term)

N° du produit ABIN7938398

L’anticorps anti-PCIF1 Polyclonal Lapin est utilisé pour la détection de PCIF1 dans des échantillons de Humain. Il a été validé pour WB et IP.

Aperçu rapide pour PCIF1 anticorps (C-Term) (ABIN7938398)

Antigène: PCIF1 (PDX1 C-terminal Inhibiting Factor 1 (PCIF1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCIF1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-PCIF1 anticorps

Épitope: C-Term

Fonction: PCIF1/CAPAM antibody (pAb)

Purification: Protein A Chromatography

Immunogène: This antibody was raised against a peptide within the C-terminal region of human PCIF1.

Isotype: IgG

Information d'application

Indications d'application: IP: 10 µL per IP WB*: 0.5-2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal IP results, the addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS, 30 % glycerol, and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur PCIF1

Antigène: PCIF1 (PDX1 C-terminal Inhibiting Factor 1 (PCIF1))

Autre désignation: PCIF1

Sujet:

Background_Plain: The abundant modification N6-methyladenosine (m6A), in eukarytic mRNAs, has been identified as a major epitranscriptome mark that plays critical roles in RNA metabolism and function. In addition to the internal m6A, N6, 2-O dimethyladenosine (m6Am) is present at the transcription start site of capped mRNAs in vertebrates. The abundance of this modification in the transcriptome has been quantified and identified as the writer protein, cap-specific adenosine methyltransferase (CAPAM/PCIF1), is needed for this modification. CAPAM/PCIF1 is identified as the Methyltransferase responsible for methylating 2-O-methyladenosine (Am) at its N6 position to generate m6Am. m6Am is involved in regulating translation of capped mRNAs. Depleting CAPAM/PCIF1 does not affect mRNA translation but is associated with reduced stability of a subset of m6Am annotated mRNAs.

Aliases: Transcription factor PCF1, Phosphorylated CTD Interacting Factor 1 (PCIF1)

Poids moléculaire: 80 kDa

NCBI Accession: NP_071387