POLR2F anticorps

N° du produit ABIN7938437

L’anticorps anti-POLR2F Monoclonal Souris est utilisé pour la détection de POLR2F dans des échantillons de Humain, Rat, Souris, C. elegans et Saccharomyces cerevisiae. Il a été validé pour WB, IF, ChIP, ChIP-seq et ICC.

Aperçu rapide pour POLR2F anticorps (ABIN7938437)

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivité: Humain, Rat, Souris, C. elegans, Saccharomyces cerevisiae

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp POLR2F est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunocytochemistry (ICC)

Clone: 4H8

Détail du produit anti-POLR2F anticorps

Fonction: RNA pol II antibody (mAb) (Clone 4H8)

Purification: Affinity Purified

Immunogène: This RNA pol II antibody was raised against a synthetic peptide YSPTSpPS corresponding to human RNA pol II. It recognizes both the phosphorylated and unphosphorylated forms of the largest subunit of RNA pol II.

Isotype: IgG1

Information d'application

Indications d'application: ChIP: 10 µL per ChIP ChIP-Seq: 6-20 µL each WB: 1:2,000 - 1:5,000 dilution For optimal results in Western blotting, primary antibody incubations should be performed overnight at 4 °C. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.2 % gelatin, 0.01 % thimerosal, 0.1 % sodium azide, and 50 % glycerol. Thimerosal and sodium azide are highly toxic.

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur POLR2F

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Autre désignation: RNA pol II

Sujet:

Background_Plain: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220

Poids moléculaire: 217 kDa

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways