POLR2F anticorps (pSer2)

N° du produit ABIN7938441

Cet anticorps anti-POLR2F Monoclonal Rat (Clone 3E10) (ABIN7938441) détecte spécifiquement POLR2F dans WB, IF, ChIP, IP, ChIP-seq et ICC. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour POLR2F anticorps (pSer2) (ABIN7938441)

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp POLR2F est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Immunocytochemistry (ICC)

Clone: 3E10

Détail du produit anti-POLR2F anticorps

Épitope: pSer2

Fonction: RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E10)

Purification: Protein G Chromatography

Immunogène: This RNA pol II CTD phospho Ser2 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 2.

Isotype: IgG1

Information d'application

Indications d'application: WB*: 0.5 - 2 μg/mL ChIP: 20 μg per ChIP ChIP-Seq: 20 μg each IF: 1:500 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For RNA pol II CTD phospho Ser2, we also offer AbFlex RNA pol II CTD phospho Ser2 Recombinant Antibody (rAb). For details, see Catalog No. 91115.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur POLR2F

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Autre désignation: RNA pol II

Sujet:

Background_Plain: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.

Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220

Poids moléculaire: 240 kDa

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways