POLR2F anticorps (meLys7)

N° du produit ABIN7938446

Cet anticorps Rat Monoclonal détecte spécifiquement POLR2F dans WB, ChIP, IP et ChIP-seq. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour POLR2F anticorps (meLys7) (ABIN7938446)

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp POLR2F est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Clone: 1F5

Détail du produit anti-POLR2F anticorps

Épitope: meLys7

Fonction: RNA pol II CTD monomethyl Lys7 antibody (mAb) (Clone 1F5)

Purification: Protein G Chromatography

Immunogène: This antibody was raised against a peptide containing dimethyl-lysine 7 of human RNA Pol II CTD.

Isotype: IgG2a

Information d'application

Indications d'application: ChIP-Seq: 10 μg per ChIP IP: 5 μg per IP WB*: 0.5 - 2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.05 % BSA and 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur POLR2F

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Autre désignation: RNA pol II

Sujet:

Background_Plain: Genome-wide profiling revealed high levels of K7me1/2 marks at the transcriptional start site of genes for sense and antisense transcribing RNAPII. The new K7 modifications further expand the mammalian CTD code to allow regulation of differential gene expression. In analogy, it appears likely that the various modifications of K7 residues identified in this analysis may similarly contribute to fine tuning of gene expressing by RNAPII, and that these modifications are placed in K7 residues of CTD dependent on cell signaling and epigenetic programming of cells.

Aliases: RNA Polymerase II Carboxy Terminal Domain

Poids moléculaire: 240 kDa

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways