POLR2F anticorps (Cit1810)

N° du produit ABIN7938448

Cet anticorps Lapin Polyclonal détecte spécifiquement POLR2F dans WB, IP et DB. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour POLR2F anticorps (Cit1810) (ABIN7938448)

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR2F est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Détail du produit anti-POLR2F anticorps

Épitope: Cit1810

Fonction: RNA pol II CTD Cit1810 antibody (pAb)

Purification: Affinity Purified

Immunogène: This RNA pol II CTD Cit 1810 antibody was raised against a peptide containing the RNA Pol II CTD sequence containing citrulline and corresponding to human RNA pol ll.

Isotype: IgG

Information d'application

Indications d'application: IP: 4 - 10 µL per IP WB: 1:500 - 1:2,000 dilution DB: 1:500 dilution For RNA pol II CTD , we also offer AbFlex RNA pol II CTD Recombinant Antibody (rAb). For details, see Catalog No. 91151.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in 70 mM Tris, 32 mM NaOAC, 0.035 % sodium azide, 30 % glycerol and 0.5 mg/mL BSA.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur POLR2F

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Autre désignation: RNA pol II

Sujet:

Background_Plain: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. This antibody is specific for the deamination of arginine and its conversation to citrulline by peptidyl arginine deiminase 2 (PADI2), an enzyme that has been associated with several diseases, including cancer. Among the PADI family members, only PADI2 citrullinates R1810 (Cit1810) at repeat 31 of the CTD. Loss of R1810 result in increased amounts of RNAP2 at transcription start cites, reduce gene expression, and inhibits cell proliferation.

Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220

Poids moléculaire: 210 kDa

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways