SUZ12 anticorps (C-Term)

N° du produit ABIN7938516

Cet anticorps anti-SUZ12 Polyclonal Lapin (ABIN7938516) détecte spécifiquement SUZ12 dans WB, IF, ChIP, ChIP-seq et CUT&RUN. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour SUZ12 anticorps (C-Term) (ABIN7938516)

Antigène: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SUZ12 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Détail du produit anti-SUZ12 anticorps

Épitope: C-Term

Fonction: Suz12 antibody (pAb)

Purification: Affinity Purified

Immunogène: This Suz12 antibody was raised against a peptide corresponding to the C-terminus of human Suz12.

Isotype: IgG

Information d'application

Indications d'application: ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB*: 1:250 - 1:1,000 dilution CUT&RUN: 1 µL per 50 µL reaction *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. An additional band at approximately 20 kDa is also detected by this antibody in HeLa and HEK lysates, but not in mouse CSML0 cell lysate. For Suz12, we also offer AbFlex Suz12 Recombinant Antibody (rAb). For details, see Catalog No. 91163.

Restrictions: For Research Use only

Stockage

Buffer: PBS, 30 % glycerol, 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur SUZ12

Antigène: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Autre désignation: Suz12

Sujet:

Background_Plain: Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3K27me2 and Histone H3K27me3), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.

Aliases: CHET9, JJAZ1

Poids moléculaire: 95 kDa

NCBI Accession: NP_056170