beta Actin anticorps (N-Term)

N° du produit ABIN7965703

Cet anticorps Lapin Polyclonal détecte spécifiquement beta Actin dans WB. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Poulet, Singe et Poisson zèbre (Danio rerio).

Aperçu rapide pour beta Actin anticorps (N-Term) (ABIN7965703)

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain, Souris, Rat, Poulet, Singe, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp beta Actin est non-conjugé

Application: Western Blotting (WB)

Classe de qualité: Carrier-free

Détail du produit anti-beta Actin anticorps

Épitope: N-Term

Fonction: Anti-beta Actin/ACTB Antibody

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross reactivity with other proteins

Homologie: identical to the related rat,mouse sequences.

Attributs du produit: Anti-beta Actin/ACTB Antibody. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Chicken, Zebrafish.

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin, identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat, Chicken, Zebrafish

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: ACTB

Sujet:

Background: beta-Actin (ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al. (1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al. (2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al. (2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.

Gene Full Name: actin beta

Sequence Similarities: Belongs to the actin family.

Poids moléculaire: 42 kDa

ID gène: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction