EIF3E anticorps (AA 160-241)

N° du produit ABIN7988876

Cet anticorps anti-EIF3E Monoclonal Souris (Clone 10F5H6) (ABIN7988876) détecte spécifiquement EIF3E dans WB et FACS. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour EIF3E anticorps (AA 160-241) (ABIN7988876)

Antigène: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EIF3E est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Classe de qualité: Carrier-free

Clone: 10F5H6

Détail du produit anti-EIF3E anticorps

Épitope: AA 160-241

Fonction: Anti-EIF3e Antibody (monoclonal, 10F5H6)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: Human EIF3e shares 100% amino acid (aa) sequence identity with both mouse,rat EIF3e.

Attributs du produit: Anti-EIF3e Antibody.(monoclonal, 10F5H6)-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human EIF3e recombinant protein (Position: A160-Q241). Human EIF3e shares 100% amino acid (aa) sequence identity with both mouse and rat EIF3e.

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur EIF3E

Antigène: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Autre désignation: EIF3E

Sujet:

Background: Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E is the component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis ts localization/assembly. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). And the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Gene Full Name: eukaryotic translation initiation factor 3 subunit E

Poids moléculaire: 52 kDa

ID gène: 3646

UniProt: P60228

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C