NSF anticorps (AA 4-636) (HRP)

N° du produit ABIN8013146

Cet anticorps Lapin Polyclonal détecte spécifiquement NSF dans WB, ELISA et IHC. Il présente une réactivité avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour NSF anticorps (AA 4-636) (HRP) (ABIN8013146)

Antigène: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NSF est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-NSF anticorps (HRP)

Épitope: AA 4-636

Fonction: Anti-NSF Antibody HRP Conjugated

Réactivité croisée (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human NSF recombinant protein (Position: R4-Q636).

Isotype: IgG

Information d'application

Indications d'application: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur NSF

Antigène: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Autre désignation: NSF

Sujet:

Background: N-ethylmaleimide-sensitive factor, also known as NSF, is an enzyme which in humans is encoded by the NSF gene. NSF is a homohexameric AAA ATPase involved in membrane fusion. NSF is ubiquitously found in the cytoplasm of eukaryotic cells. It is a central component of the cellular machinery in the transfer of membrane vesicles from one membrane compartment to another. During this process, SNARE proteins on two joining membranes (usually a vesicle and a target membrane such as the plasma membrane) form a tight complex. This aids fusion of the vesicle with the target membrane. It has been proposed that the role of NSF is to undo these SNARE complexes once membrane fusion has occurred, using the hydrolysis of ATP as an energy source, allowing the dissociated SNAREs to be recycled for reuse in further rounds of membrane fusion. This proposal remains controversial, however. Recent work indicates that the ATPase function of NSF does not function in recycling of vesicles but rather functions in the act of fusing vesicles with the plasma membrane.

Gene Full Name: N-ethylmaleimide sensitive factor, vesicle fusing ATPase

ID gène: 4905

UniProt: P46459