N4BP1 anticorps (AA 1-885) (HRP)

N° du produit ABIN8014125

L’anticorps anti-N4BP1 Polyclonal Lapin est utilisé pour la détection de N4BP1 dans des échantillons de Humain. Il a été validé pour WB, ELISA et IHC.

Aperçu rapide pour N4BP1 anticorps (AA 1-885) (HRP) (ABIN8014125)

Antigène: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp N4BP1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-N4BP1 anticorps (HRP)

Épitope: AA 1-885

Fonction: Anti-N4BP1 Antibody HRP Conjugated

Réactivité croisée (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human N4BP1 recombinant protein (Position: M1-K885).

Isotype: IgG

Information d'application

Indications d'application: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur N4BP1

Antigène: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Autre désignation: N4BP1

Sujet:

Background: Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73α, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73α for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73α and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.

Gene Full Name: NEDD4 binding protein 1

ID gène: 9683

UniProt: O75113