NFIB anticorps (Middle Region)

N° du produit ABIN8016396

L’anticorps anti-NFIB Monoclonal Souris est utilisé pour la détection de NFIB dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ICC, IF et FACS.

Aperçu rapide pour NFIB anticorps (Middle Region) (ABIN8016396)

Antigène: NFIB (Nuclear Factor I/B (NFIB))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NFIB est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Carrier-free

Clone: 4D6E4

Détail du produit anti-NFIB anticorps

Épitope: Middle Region

Fonction: Anti-NFIB/NF1B2 Antibody (monoclonal, 4D6E4)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: identical to the related mouse,rat sequences.

Attributs du produit: Anti-NFIB/NF1B2 Antibody.(monoclonal, 4D6E4)-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human NFIB/NF1B2, identical to the related mouse and rat sequences.

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur NFIB

Antigène: NFIB (Nuclear Factor I/B (NFIB))

Autre désignation: NFIB

Sujet:

Background: Nuclear factor 1 B-type is a protein that in humans is encoded by the NFIB gene. The NFIB gene is a part of the NFI gene complex that includes three other genes (NFIA, NFIC and NFIX). The NFIB gene is a protein coding gene that also serves as a transcription factor. This gene is essential in embryonic development and it works together with its gene complex to initiate tissue differentiation in the fetus. Through knockout experiments, researchers found that mice without the NFIB gene have severely underdeveloped lungs. This mutation does not seem to cause spontaneous abortions because in utero the fetus does not use its lungs for respiration. However, this becomes lethal once the fetus is born and has to take its first breath. It is thought that NFIB plays a role in down regulating the transcription factors TGF-β1 and Shh in normal gestation because they remained high in knockout experiments. The absence of NFIB also leads to insufficient amounts of surfactant being produced which is one reason why the mice cannot breathe once it is born. The knockout experiments demonstrated that NFIB has a significant role in fore-brain development. NFIB is typically found in pontine nuclei of the CNS, the cerebral cortex and the white matter of the brain and without NFIB these areas are dramatically affected.

Gene Full Name: nuclear factor I B

Poids moléculaire: 68 kDa

ID gène: 4781

UniProt: O00712