SPARC anticorps (AA 107-303)

N° du produit ABIN8035174

Cet anticorps anti-SPARC Polyclonal Lapin (ABIN8035174) détecte spécifiquement SPARC dans WB. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour SPARC anticorps (AA 107-303) (ABIN8035174)

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SPARC est non-conjugé

Application: Western Blotting (WB)

Classe de qualité: Carrier-free

Détail du produit anti-SPARC anticorps

Épitope: AA 107-303

Fonction: Anti-SPARC Antibody

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: Human SPARC shares 97% amino acid (aa) sequence identity with both mouse,rat SPARC.

Attributs du produit: Anti-SPARC Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human SPARC recombinant protein (Position: E107-I303). Human SPARC shares 97% amino acid (aa) sequence identity with both mouse and rat SPARC.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur SPARC

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Autre désignation: SPARC

Sujet:

Background: SPARC, secreted protein acidic and rich in cysteine, also known as Osteonectin is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Furthermore, SPARC is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. SPARC also shows affinity for collagen in addition to bone mineral calcium. A correlation between SPARC over expression and ampullary cancers and chronic pancreatitis has been found.

Gene Full Name: secreted protein acidic and cysteine rich

Poids moléculaire: 35 kDa

ID gène: 6678

UniProt: P09486

Pathways: Autophagy