C5b-9 anticorps

N° du produit ABIN8065530

L’anticorps anti-C5b-9 Monoclonal Souris est utilisé pour la détection de C5b-9 dans des échantillons de Humain. Il a été validé pour ELISA.

Aperçu rapide pour C5b-9 anticorps (ABIN8065530)

Antigène: C5b-9 (Terminal Complement Complex C5b-9 (C5b-9))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp C5b-9 est non-conjugé

Application: ELISA

Classe de qualité: Carrier-free

Clone: AE11

Détail du produit anti-C5b-9 anticorps

Fonction: Anti-Complement C5b-9 Antibody

Specificité: DIA 011-01 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive). (1) DIA 011-01 binds to a neoepitope exposed on C9

Homologie: Horse,Human,Non-Human Primate,and Pig

Purification: Protein A or Protein G purified

Immunogène: Purified C5b-9

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: 2-8°C without exposure to light

Date de péremption: 12 months

Détails sur C5b-9

Antigène: C5b-9 (Terminal Complement Complex C5b-9 (C5b-9))

Autre désignation: Complement C5b-9

Sujet: C5, C5D, C5a, C5b, CPAMD4, ECLZB, complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, C5a anaphylatoxin, anaphylatoxin C5a analog, complement component 5, prepro-C5,Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:12878586, PubMed:18204047, PubMed:30643019, PubMed:6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed:12878586, PubMed:18204047, PubMed:30643019, PubMed:39914456, PubMed:39814882, PubMed:6554279). Complement C5b Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed:26841837, PubMed:27052168, PubMed:30552328, PubMed:30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex: C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed:30552328, PubMed:30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed:30552328, PubMed:32569291).,C5, C6, C7, C8, C9,C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and DIA 011-01 is directed against a neoepitope exposed on C9 when incorporated into the TCC.

ID gène: 727

UniProt: P01031, P13671, P06681, P07357, P07358, P07360, P02749