Recombinant SARS-CoV-2 Spike S1 anticorps (AA 319-591)

N° du produit ABIN8071383

L’anticorps anti-SARS-CoV-2 Spike S1 Monoclonal Souris est utilisé pour la détection de SARS-CoV-2 Spike S1 dans des échantillons de SARS Coronavirus-2 (SARS-CoV-2). Il a été validé pour ELISA, WB et ICC.

Aperçu rapide pour Recombinant SARS-CoV-2 Spike S1 anticorps (AA 319-591) (ABIN8071383)

Antigène: SARS-CoV-2 Spike S1

Type d'anticorp: Recombinant Antibody

Reactivité: SARS Coronavirus-2 (SARS-CoV-2)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SARS-CoV-2 Spike S1 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC)

Clone: AB68-A09

Détail du produit anti-SARS-CoV-2 Spike S1 anticorps

Épitope: AA 319-591

Fonction: anti-SARS-CoV-2 Spike Protein S1 (RBD), mAb (rec.) (AB68-A09) (Fc Mouse)

Specificité: Recognizes the SARS-CoV-2 S1 (Receptor-binding domain). Does not cross-react with HCoV-OC43, HCoV-229E, HCoV-NL63, HCoV-HKU1, MERS-CoV or SARS-CoV.

Attributs du produit: Recombinant Antibody. Recognizes the SARS-CoV-2 S1 (Receptor-binding domain). Does not cross-react with HCoV-OC43, HCoV-229E, HCoV-NL63, HCoV-HKU1, MERS-CoV or SARS-CoV. Applications: ELISA, ICC, WB. Clone: AB68-A09. Isotype: Mouse IgG2a. Formulation: Liquid. In PBS. Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome beta-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 shares 79.5 % sequence identity with SARS-CoV and is 96.2 % identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S protein plays a key role in viral infection and pathogenesis. The S1 subunit contains the N-terminal domain (NTD) and a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells, whereas S2 harbors heptad repeat 1 (HR1) and HR2. The RBD domain first binds its receptor to form an RBD/ACE2 complex. This triggers conformational changes in the S protein, leading to membrane fusion mediated via HR1 and HR2 and consequently in viral entry into target cells. Antibodies targeting various regions of S protein have different mechanisms in inhibiting SARS-CoV-2 infection. For example, NTD-targeting antibodies bind the NTD to form an NTD/mAb complex, thereby preventing conformational changes in the S protein and blocking membrane fusion and viral entry. RBD-targeting antibodies form RBD/mAb or RBD/Nb complexes that inhibit binding of the RBD to ACE2, thereby preventing entry of SARS-CoV-2 into target cells.

Pureté: >95 % (SDS-PAGE)

Immunogène: Recombinant SARS-CoV-2 S1 receptor binding protein (aa 319-591) containing a C-terminal human Fc-tag.

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS.

Conseil sur la manipulation: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Please handle under sterile conditions to avoid contamination.

Stock: 4 °C,-20 °C

Stockage commentaire:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.Stable for at least 3 months after receipt when stored at +4°C.

Date de péremption: 12 months

Détails sur SARS-CoV-2 Spike S1

Antigène: SARS-CoV-2 Spike S1

Autre désignation: SARS-CoV-2 Spike Protein S1

Classe de substances: Viral Protein

Sujet:

Alternate Names/Synonyms: 2019-nCoV Spike Protein S1 (RBD), Spike Receptor Binding Domain

Product Description: Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome beta-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 shares 79.5 % sequence identity with SARS-CoV and is 96.2 % identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S protein plays a key role in viral infection and pathogenesis. The S1 subunit contains the N-terminal domain (NTD) and a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells, whereas S2 harbors heptad repeat 1 (HR1) and HR2. The RBD domain first binds its receptor to form an RBD/ACE2 complex. This triggers conformational changes in the S protein, leading to membrane fusion mediated via HR1 and HR2 and consequently in viral entry into target cells. Antibodies targeting various regions of S protein have different mechanisms in inhibiting SARS-CoV-2 infection. For example, NTD-targeting antibodies bind the NTD to form an NTD/mAb complex, thereby preventing conformational changes in the S protein and blocking membrane fusion and viral entry. RBD-targeting antibodies form RBD/mAb or RBD/Nb complexes that inhibit binding of the RBD to ACE2, thereby preventing entry of SARS-CoV-2 into target cells.