TUBB anticorps

N° du produit ABIN8071385

Cet anticorps Souris Monoclonal détecte spécifiquement TUBB dans WB, IHC, ICC et IP. Il présente une réactivité avec des échantillons de Humain, Souris et Paramecium.

Aperçu rapide pour TUBB anticorps (ABIN8071385)

Antigène: TUBB (Tubulin, beta (TUBB))

Reactivité: Humain, Souris, Paramecium

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: AXO45

Détail du produit anti-TUBB anticorps

Fonction: anti-beta-Tubulin, mAb (AXO45)

Specificité: Detects almost all isotypes of beta-tubulin specifically in cells and tissues of mouse, human and ciliates.

Attributs du produit: Monoclonal Antibody. Detects almost all isotypes of β-tubulin specifically in cells and tissues of mouse, human and ciliates. Applications: ICC, IHC, IP and WB. Clone: AXO45. Isotype: Mouse IgG1. The β-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both α- and β-tubulin (A.M. Callen, et al., 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of β-tubulin. Although the antibody was raised against the β-tubulin found in cilia, it is able to recognize β-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of β-tubulin found in mammals.

Pureté: >95 % (SDS-PAGE)

Immunogène: Axonemal tubulin from the ciliate Paramecium electroeluted from the 55 kDa band of the SDS-PAGE gel.

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire:

+4°C

-20°C

Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Détails sur TUBB

Antigène: TUBB (Tubulin, beta (TUBB))

Autre désignation: beta-Tubulin

Sujet:

Alternate Names/Synonyms: Tubulin beta Chain, Tubulin beta-5 Chain

Product Description: The beta-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both alpha- and beta-tubulin (A.M. Callen, et al., 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of beta-tubulin. Although the antibody was raised against the beta-tubulin found in cilia, it is able to recognize beta-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of beta-tubulin found in mammals.

Pathways: Dynamique des Microtubules, M Phase