CD200 anticorps (AA 41-140)

N° du produit ABIN761396

Détail du produit anti-CD200 anticorps

Antigène: CD200

Épitope: AA 41-140

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD200 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Réactivité croisée: Humain

Homologie: Mouse,Rat

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human CD200

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #101517 (Immunohistochemistry)

by: Department of Pathology and Molecular Medicine, McMaster University

No.: #101517

Date: 11.07.2017

Antigène: CD200

Numéro du lot: AG06145442

Application validée: Immunohistochemistry

Contrôle positif: Human 7 week normal karyotype elective termination placenta

Contrôle négative: Placental villus from 7w gestation karyotype-normal pregnancy, stained with KLH antibody ABIN401183

Conclusion:

Passed. ABIN761396 specifically labels the CD200 in human placental tissue.

Validation Images

Staining with ABIN761396 at dilutions 1:200, 1:250, 1:300, and 1:400 (A, B, C, and D) using a 7 week elective termination of a pregnancy that tested negative for trisomies (Clark et al. (2017) Am. J. Reprod. Immunol). Optimal staining was obtained at 1:200 dilution. Anti-KLH control (E) at 1:200 was negative.

Protocole

Anticorps primaire: ABIN761396

Anticorps secondaire: Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232)

Full Protocol:

• Fix human placental tissue in 10% buffered formalin for 24h at RT.
• Process and embed tissue in paraffin.
• Cut paraffin blocks with a Leica CM2255 Microtome into 4µm sections.
• Affix sections to positively charged slides and air dry ON at RT.
• Deway and hydrate the slides on an automated Leica BOND Rx stainer.
• Antigen retrieval on the Leica BOND Rx automated stainer using epitope retrieval buffer 2 (Leica, AR9640, lot ER20172).
• Stain slides with primary
• • Rabbit anti-CD200 antibody (AA 45-95) antibody (antibodies-online, ABIN761396, lot 980502W),
  • rabbit anti-CD200 Receptor 1-Like (CD200R1L) (AA 150-200) antibody(antibodies-online, ABIN1715098, lot 9A13M60), or
  • rabbit anti-KLH antibody (antibodies-online, ABIN401183, lot 304770)
• diluted 1:200 in Power Vision IHC Super Blocker (Leica, PV6122). The staining protocol incorporates a modified Leica standard protocol IHC-F (which omits the post-primary step) and uses the standard times outlined in the machine protocol.
• Stain sections with Bond Polymer Refine Detection kit (Leica, DS9800, lot 49232) containing peroxidase block, post primary antibody, polymer as well as DAB chromogen and hematoxylin counterstain for times outlined in the standard protocol IHC-F.
• Remove slides from the Leica Bond Rx and then dehydrate in ethanol and clear in xylene.
• Mount slides in permount mounting medium (Fisher Scientific, SP15-500, lot 162767).
• After the slide-coverslip edges are dry, scan slides using Imagescope and photograph at 400x.

Notes:

• Several dilutions from 1:200 to 1:400 of ABIN761396 were tested and 1:200 was found to be optimal.

• The staining pattern of ABIN761396 matches our results with the rabbit anti-CD200 polyclonal antibody RB846 and observations made by others. It is also consistent with staining with the CD200R1L antibody ABIN1715098).

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-CD200 (ABIN761396)

Clark, Arredondo, Dhesy-Thind: "The CD200 tolerance-signaling molecule and its receptor, CD200R1, are expressed in human placental villus trophoblast and in peri-implant decidua by 5 weeks' gestation." dans: Journal of reproductive immunology, Vol. 112, pp. 20-3, (2015) (PubMed).

Détails sur CD200

Antigène: CD200

Autre désignation: Cd200 (CD200 Produits)

Synonymes: anticorps MOX1, anticorps MOX2, anticorps MRC, anticorps OX-2, anticorps CD200, anticorps Cspmo2, anticorps MRCOX2, anticorps Mox2, anticorps DKFZp459C0716, anticorps OX2, anticorps CD200 molecule, anticorps Cd200 molecule, anticorps CD200 antigen, anticorps CD200, anticorps Cd200

Sujet:

Synonyms: MRC, MOX1, MOX2, OX-2, OX-2 membrane glycoprotein, CD200, My033

Background: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.

ID gène: 4345

UniProt: P41217