PROL1 anticorps (Internal Region)

N° du produit ABIN768578

L’anticorps Chèvre Polyclonal anti-PROL1 a été validé pour WB, IHC, IP, ELISA et IF. Il convient pour détecter PROL1 dans des échantillons de Souris. Il y a 2+ publications disponibles.

Aperçu rapide pour PROL1 anticorps (Internal Region) (ABIN768578)

Antigène: PROL1 (Proline Rich, Lacrimal 1 (PROL1))

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PROL1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-PROL1 anticorps

Épitope: Internal Region

Fonction: Mucin 10 / Prol1

Séquence: QFPVRKYLED PRY

Réactivité croisée: Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QFPVRKYLEDPRY, from the internal region of the protein sequence according to NP_032670.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Positive staining in the submandibular salivary gland of the mouse, while cells remain negative in the sublingual salivary gland. Data provided by Everest Grant winner Melinda Larsen State University of New York, Albany, NY. A publi

Western Blot: This antibody has been successfully used in WB on Mouse: Peluso G et al. (2019) J Biol Chem. 2019 Dec 27. pii: jbc.RA119.009807. PMID: 31882545.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: This antibody has been successfully used in IF on Mouse: Peluso G et al. (2019) J Biol Chem. 2019 Dec 27. pii: jbc.RA119.009807. PMID: 31882545.

Immunoprecipitation: This antibody has been successfu

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-PROL1 (ABIN768578)

Tanaka, Ogawa, Hojo, Kawashima, Mabuchi, Hata, Nakamura, Yasuhara, Takamatsu, Irié, Fukada, Sakai, Inoue, Nishimura, Ohara, Saito, Ohba, Tsuji, Mishima: "Generation of orthotopically functional salivary gland from embryonic stem cells." dans: Nature communications, Vol. 9, Issue 1, pp. 4216, (2019) (PubMed).

Nelson, Manhardt, Kamath, Sui, Santamaria-Pang, Can, Bello, Corwin, Dinn, Lazare, Gervais, Sequeira, Peters, Ginty, Gerdes, Larsen: "Quantitative single cell analysis of cell population dynamics during submandibular salivary gland development and differentiation." dans: Biology open, Vol. 2, Issue 5, pp. 439-47, (2013) (PubMed).

Détails sur PROL1

Antigène: PROL1 (Proline Rich, Lacrimal 1 (PROL1))

Autre désignation: Prol1

Sujet: Prol1, proline rich, lacrimal 1, Muc10, mucin 10, mucin 10, submandibular gland salivary mucin

Poids moléculaire: 45kDa

ID gène: 17830

NCBI Accession: NP_032670