His Tag anticorps

N° du produit ABIN781760

L’anticorps anti-His Tag Monoclonal Souris est utilisé pour la détection de His Tag. Il a été validé pour WB et EIA.

Aperçu rapide pour His Tag anticorps (ABIN781760)

Antigène: His Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 16-002

Détail du produit anti-His Tag anticorps

Séquence: HHHHHH

Purification: Affinity Chromatography on Protein G

Immunogène: Purified, recombinant C-terminal His6-tagged protein expressed in E. coli

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Upon receipt, store (in aliquots) at -20 °C to -70 °C.

Détails sur His Tag

Antigène: His Tag

Autre désignation: 6xHistidine Epitope Tag

Classe de substances: Tag

Sujet: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag