Tryptophan Hydroxylase 2 anticorps (N-Term)

N° du produit ABIN784279

Cet anticorps Chèvre Polyclonal détecte spécifiquement Tryptophan Hydroxylase 2 dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache) et Porc.

Aperçu rapide pour Tryptophan Hydroxylase 2 anticorps (N-Term) (ABIN784279)

Antigène: Tryptophan Hydroxylase 2 (TPH2)

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Tryptophan Hydroxylase 2 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Tryptophan Hydroxylase 2 anticorps

Épitope: AA 16-29, N-Term

Séquence: RGFSLDSAVP EEHQ-C

Specificité: Recognizes TPH2 (16-29).

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence from the N Terminus of the protein sequence according to NP_775489.2. Genename: TPH2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Tryptophan Hydroxylase 2

Antigène: Tryptophan Hydroxylase 2 (TPH2)

Autre désignation: Tryptophan 5-Hydroxylase 2 (TPH2)

Sujet: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).Synonyms: NTPH, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2

ID gène: 121278

NCBI Accession: NP_775489