GIT1 anticorps (pTyr545) (HRP)

N° du produit ABIN801117

Cet anticorps anti-GIT1 est un anticorps Lapin Polyclonal détectant GIT1 dans WB, ELISA, IHC (p) et IHC (fro). Adapté pour Humain.

Aperçu rapide pour GIT1 anticorps (pTyr545) (HRP) (ABIN801117)

Antigène: GIT1

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GIT1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-GIT1 anticorps (HRP)

Épitope: pTyr545

Homologie: Human,Mouse,Rat,Cow,Pig

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human GIT1 around the phosphorylation site of Tyr545

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur GIT1

Antigène: GIT1

Autre désignation: GIT1

Sujet:

Synonyms: GIT1 phospho Tyr545, p-GIT1 Tyr545, ARF GAP GIT1, ARF GTPase activating protein GIT1, CAT 1, Cat1, Cool associated and tyrosine phosphorylated protein 1, G protein coupled receptor kinase interactor 1, GRK interacting protein 1.

Background: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist (1,2). G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade (3,4). GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking (5,6). GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, thus reducing b2-adrenergic receptor signaling (5).

ID gène: 28964