SLC9A3 anticorps

N° du produit ABIN863199

Cet anticorps anti-SLC9A3 est un anticorps Lapin Polyclonal détectant SLC9A3 dans WB, IHC, ICC et IF. Adapté pour Rat. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour SLC9A3 anticorps (ABIN863199)

Antigène: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC9A3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-SLC9A3 anticorps

Specificité: Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level),Detects ~84 kDa.

Réactivité croisée: Souris, Rat

Purification: Protein A Purified

Immunogène: Synthetic peptide mapping to AA 809 to 831 of rat sequence

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN863199 was sufficient for detection of HNE3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-SLC9A3 (ABIN863199)

Jones, Bailey, Murray, Lu, McNeilly, Schlötzer-Schrehardt, Lennon, Sado, Brownstein, Mullins, Kadler, Van Agtmael: "ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice." dans: Disease models & mechanisms, Vol. 9, Issue 2, pp. 165-76, (2016) (PubMed).

Lee, Osis, Handlogten, Guo, Verlander, Weiner: "Effect of dietary protein restriction on renal ammonia metabolism." dans: American journal of physiology. Renal physiology, Vol. 308, Issue 12, pp. F1463-73, (2015) (PubMed).

Bourgeois, Bounoure, Christensen, Ramakrishnan, Houillier, Devuyst, Wagner: "Haploinsufficiency of the ammonia transporter Rhcg predisposes to chronic acidosis: Rhcg is critical for apical and basolateral ammonia transport in the mouse collecting duct." dans: The Journal of biological chemistry, Vol. 288, Issue 8, pp. 5518-29, (2013) (PubMed).

Détails sur SLC9A3

Antigène: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Autre désignation: NHE3

Sujet: Sodium-hydrogen exchanger 3 (NHE3, Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).

ID gène: 24784

NCBI Accession: NP_036786

UniProt: P26433

Pathways: Proton Transport