PKM1 (AA 399-403) anticorps

N° du produit ABIN871702

Détail du produit

Antigène: PKM1

Épitope: AA 399-403

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Specificité: The antibody detects endogenous levels of total PKM1 protein.

Purification: Affinity purified

Immunogène: Peptide sequence around AA 399-403 (V-R-A-S-S) derived from Human PKM1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Information d'application

Indications d'application: Western blotting: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Référence

Jiang, Li, Yang, Hawke, Zheng, Xia, Aldape, Wei, Guo, Chen, Lu: "PKM2 regulates chromosome segregation and mitosis progression of tumor cells." dans: Molecular cell, Vol. 53, Issue 1, pp. 75-87, (2014) (PubMed).

Yang, Xia, Hawke, Li, Liang, Xing, Aldape, Hunter, Alfred Yung, Lu: "PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis." dans: Cell, Vol. 150, Issue 4, pp. 685-96, (2012) (PubMed).

Détail du antigène

Antigène: PKM1

Sujet: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Poids moléculaire: 60 kDa

ID gène: 5315

NCBI Accession: NP_872270

UniProt: P14618