Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CCDC42 anticorps (AA 221-312)

CCDC42 Reactivité: Souris, Rat WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN872760
  • Antigène Voir toutes CCDC42 Anticorps
    CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))
    Épitope
    • 14
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 1
    • 1
    AA 221-312
    Reactivité
    • 24
    • 23
    • 16
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris, Rat
    Hôte
    • 38
    Lapin
    Clonalité
    • 38
    Polyclonal
    Conjugué
    • 8
    • 5
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CCDC42 est non-conjugé
    Application
    • 32
    • 21
    • 14
    • 12
    • 12
    • 3
    • 2
    Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
     Réactivité croisée
    Souris, Rat
    Homologie
    Human,Dog,Cow,Sheep,Pig,Horse,Rabbit
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human CCDC42
    Isotype
    IgG
  • Indications d'application
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #103396 (Immunocytochemistry)
    'Independent Validation' signe
    by
    Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen
    No.
    #103396
    Date
    12.10.2018
    Antigène
    CCDC42
    Numéro du lot
    AH09037940
    Application validée
    Immunocytochemistry
    Contrôle positif
    NIH/3T3 cells transfected with Ccdc42-mCherry expression vector
    Contrôle négative
    NIH/3T3 cell lysates
    Conclusion

    ABIN872760 specifically detects exogenously expressed Ccdc42-Cherry but shows also unspecific background staining.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN872760 
    Anticorps secondaire
    goat anti-rabbit 488 (MoBiotec MFP-A1008, lot 2806F058)
    Full Protocol
    • Grow NIH/3T3 cells (ATCC, CRL-1658) in DMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco 15140-122, lot 1924797), at 37°C and 5% CO2 to 70% confluency.
    • Transfect cells with mCherry-tagged Ccdc42 (Ccdc42-Cherry) expression vector using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
    • Grow cells for 24h.
    • Fix cells in methanol for 10min at -20°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
    • Block cells in PBS containing 1% bovine serum albumin and 0.5% Tween-20 (PBT) for 1h at RT.
    • Incubate cells with primary rabbit anti-CCDC42 antibody (antibodies-online, ABIN872760, lot AH09037940) diluted 1:100 in PBS for 1h at 37°C.
    • Wash cells 3x 5min with 1x PBS.
    • Incubate cells with secondary goat anti-rabbit 488 antibody (MoBiotec MFP-A1008, lot 2806F058) diluted 1:300 in PBS for 1h at 37°C.
    • DNA counterstained with DAPI (4’,6-Diamidino-2-phenylindole; Sigma D-9542).
    • Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.
    Notes
  • Validation #103397 (Western Blotting)
    'Independent Validation' signe
    by
    Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen
    No.
    #103397
    Date
    12.10.2018
    Antigène
    CCDC42
    Numéro du lot
    AH09037940
    Application validée
    Western Blotting
    Contrôle positif
    Lysate from HEK293 cells transfected with mCherry tagged Ccdc42 expression vector
    Contrôle négative
    Lysate from untransfected HEK293 cells
    Conclusion

    Passed. ABIN872760 specifically detects Ccdc42 by immunoblotting.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN872760 
    Anticorps secondaire
    goat anti-rabbit IgG (H+L), HRP-conjugated (Jackson Immuno Research, 111-035-003, lot 123450)
    Full Protocol
    • Grow HEK293 (ATCC) cells in DMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco, 15140-122, lot 1924797), at 37°C and 5% CO2 to 70% confluency.
    • Transfect cells with mCherry tagged Ccdc42 (Ccdc42-Cherry) expression vector using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
    • Grow cells for 24h.
    • Lyse cells in SDS-sample buffer and denature total cellular lysates for 5min at 95°C.
    • Separate up to 40µl of protein lysate on a denaturing 10% SDS polyacrylamide gel (Laemmli (1970)).
    • Transfer proteins onto 0.2µm Protran membrane (GE Healthcare, 10600004, A10043108) with a Western blotting system for 1h at 400A (Towbin et al. (1979)).
    • Block the membrane in TBST (50mM Tris-HCl pH7.4, 150mM NaCl, 0.2% Tween 20) containing 5% dry milk (blocking solution) for 1h at RT.
    • Incubate membrane with primary
      • rabbit anti-Ccdc42 antibody (antibodies-online, ABIN872760, lot AH09037940) diluted 1:500 in blocking solution ON at 4°C or
      • rat anti-RFP antibody (antibodies-online, ABIN334653, lot 090428) diluted 1:500 in blocking solution ON at 4°C.
    • Wash membrane for 6x 5min with TBST at RT.
    • Incubate membrane with secondary
      • goat anti-rabbit IgG (H+L), HRP-conjugated antibody (Jackson Immuno Research, 111-035-003, lot 123450) diluted 1:2000 in blocking solution for 45min at RT or
      • rabbit anti-rat IgG-HRPconjugated antibody (Jackson ImmunoResearch Lab. Inc., 312-035-003, lot 34336) diluted 1:2000 in blocking solution for 45min at RT.
    • Wash membranes for 45 min at RT with TBST.
    • Reveal protein bands using Clarity Max Western ECL substrate (Bio-Rad, 1705062) and capture images via Chemidoc Imaging System (Bio-Rad).
    Notes

    Expected molecular mass of Ccdc42-tagged Cherry is approximately 68kDa. ABIN872760 detects a protein in the expected molecular mass range. The anti-RedFP antibody detects a protein with a similar mass in addition to proteins with lower molecular masses, which are most probably degradation products.

  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Date de péremption
    12 months
  • Antigène
    CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))
    Autre désignation
    CCDC42 (CCDC42 Produits)
    Synonymes
    anticorps RGD1565925, anticorps CCDC42A, anticorps A530001H01Rik, anticorps coiled-coil domain containing 42, anticorps Ccdc42, anticorps CCDC42
    Sujet

    Synonyms: CCDC42A, Coiled coil domain containing 42, Coiled coil domain containing protein 42A, FLJ32734, OTTMUSP00000006142, RGD1565925, RP23-400O5.1, CCD42_HUMAN.

    Background: The coiled-coil domain is a structural motif found in proteins that are involved in a diverse array of biological functions such as the regulation of gene expression, cell division, membrane fusion and drug extrusion and delivery. CCDC42 (coiled-coil domain containing 42) is a 316 amino acid protein encoded by a gene that maps to human chromosome 17p13.1. Encoding over 1,200 genes, chromosome 17 comprises over 2.5 % of the human genome. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome.

    ID gène
    146849
Vous êtes ici:
Support technique