CDT2/RAMP anticorps (AA 181-280)

N° du produit ABIN873173

Détail du produit anti-CDT2/RAMP anticorps

Antigène: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Épitope: AA 181-280

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDT2/RAMP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Homologie: Human,Mouse,Rat,Dog,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human RAMP/CDT2

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur CDT2/RAMP

Antigène: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Autre désignation: CDT2 (DTL Produits)

Synonymes: anticorps CDT2, anticorps DCAF2, anticorps L2DTL, anticorps RAMP, anticorps 2810047L02Rik, anticorps 5730564G15Rik, anticorps Ramp, anticorps RGD1310439, anticorps cdt2, anticorps cdt2-a, anticorps dcaf2, anticorps l2dtl, anticorps ramp, anticorps cb151, anticorps chunp6871, anticorps wu:fb54b02, anticorps denticleless E3 ubiquitin protein ligase homolog, anticorps denticleless E3 ubiquitin protein ligase, anticorps denticleless E3 ubiquitin protein ligase homolog S homeolog, anticorps denticleless E3 ubiquitin protein ligase homolog (Drosophila), anticorps DTL, anticorps Dtl, anticorps dtl.S, anticorps dtl

Sujet:

Synonyms: Lethal2 denticleless protein homolog, CDW1, CDW1, DCAF2, DDB1 and CUL4 associated factor 2, Ddb1- and Cul4-associated factor 2, Denticleless homolog, Denticleless homolog Drosophila, Denticleless protein homolog, Dtl, DTL_HUMAN, L2DTL, Lethal2 denticleless protein homolog, RA regulated nuclear matrix associated protein, RAMP, Retinoic acid regulated nuclear matrix associated protein, Retinoic acid-regulated nuclear matrix-associated protein.

Background: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.Tissue specificity: Expressed in placenta and testis, very low expression seen in skeletal muscle. Detected in all hematopoietic tissues examined, with highest expression in thymus and bone marrow. A low level detected in the spleen and lymph node, and barely detectable level in the peripheral leukocytes. RA treatment down-regulated the expression in NT2 cell.

ID gène: 51514