PNPT1 anticorps (AA 551-650) (HRP)

N° du produit ABIN911632

Cet anticorps Lapin Polyclonal détecte spécifiquement PNPT1 dans WB, ELISA, IHC (p) et IHC (fro). Il présente une réactivité envers Rat.

Aperçu rapide pour PNPT1 anticorps (AA 551-650) (HRP) (ABIN911632)

Antigène: PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PNPT1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-PNPT1 anticorps (HRP)

Épitope: AA 551-650

Réactivité croisée: Rat

Homologie: Human,Mouse,Dog,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human PNPT1

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur PNPT1

Antigène: PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))

Autre désignation: PNPT1

Sujet:

Synonyms: OLD35, DFNB70, PNPASE, old-35, COXPD13, Polyribonucleotide nucleotidyltransferase 1, mitochondrial, 3'-5' RNA exonuclease OLD35, PNPase old-35, Polynucleotide phosphorylase 1, PNPase 1, Polynucleotide phosphorylase-like protein, PNPT1

Background: RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration, regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity, degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells, specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.

ID gène: 87178

UniProt: Q8TCS8