P-Glycoprotein anticorps

N° du produit ABIN920703

Cet anticorps anti-P-Glycoprotein est un anticorps Souris Monoclonal détectant P-Glycoprotein dans WB, IHC, FACS et ICC. Adapté pour Humain et Hamster.

Aperçu rapide pour P-Glycoprotein anticorps (ABIN920703)

Antigène: P-Glycoprotein

Reactivité: Humain, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp P-Glycoprotein est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: JSB-1

Détail du produit anti-P-Glycoprotein anticorps

Specificité: JSB-1 reacts with a conserved cytoplasmic epitope of the human plasma membrane- associated 170-180 kDa glycoprotein, the expression of which is strongly correlated with the degree of multidrug resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas.

Isotype: IgG1

Information d'application

Indications d'application: Can be used for the detection of MDR cells in human tumor samples. Flow Cytometry: Use at least 1:10. Fix cells in 10% (v/v) Lysing Solution, followed by primary antibody and anti-mouse-FITC. Immunocytochemistry: Use at least 1:20 (acetone fixed cell preparation) Immunohistochemistry: acetone-fixed frozen sections or formalin-fixed paraffin embedded tissues: Use at 1:20 dilution. Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In case the B-5 fixative is used, paraffin-embedded tissue can also be stained with the antibody. Western blotting: Start optimizing working dilution at 1:10, followed by incubation with anti-mouse HRP.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 1 ml concentrated tissue culture supernatant (200 tests) at ~250 µg IgG/ml, with 1% BSA and 0.1% NaN3.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur P-Glycoprotein

Antigène: P-Glycoprotein