Vimentin anticorps

N° du produit ABIN920739

Cet anticorps anti-Vimentin Monoclonal Souris (Clone V9) (ABIN920739) détecte spécifiquement Vimentin dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Vimentin anticorps (ABIN920739)

Antigène: Vimentin (VIM)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: V9

Détail du produit anti-Vimentin anticorps

Specificité: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells, and has a molecular weight of 57 kDa. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments. The antibody stains the 57 kDa vimentin band in immunoblots performed on a lysate of normal human lymphocytes. No detectable cross-reactivity with GFAP, Keratin, Neurofilament or Desmin.

Purification: Purified

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry: For use on frozen sections and formalin-fixed/paraffin- embedded tissues if pre-treated with trypsin. Paraffin sections: Use at an approximate dilution of 1:50 - 1:100 in PBS. Trypsin digestion may be required. Frozen sections: Use at an approximate dilution of 1:300 in PBS.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 1 mL of purified monoclonal antibody at approximately 30 µg/mL in PBS containing 20 mM sodium azide, and protein stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Pathways: Caspase Cascade in Apoptosis