E Tag anticorps
Aperçu rapide pour E Tag anticorps (ABIN920773)
Antigène
Hôte
Clonalité
Conjugué
Application
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Séquence
- GAPVPYPDPL EPR
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Purification
- Affinity purified
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Indications d'application
- Western blot: Recommend dilution range 1:1,000 - 1:3,000
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- PBS pH 7.2 with 0.1% sodium azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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- E Tag
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Autre désignation
- E-tag
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Classe de substances
- Tag
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Sujet
- Rabbits were immunized with E-tag peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid phase. Specification: ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA (E-tag-BSA). A 1:10,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP-conjugated donkey anti-rabbit (IgG) at 1:18,000 and TMB as the substrate. Appropriate specificity controls were run.
Antigène
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