50 mM HEPES pH 7.0, 0.1 M sodium chloride with 0.2% BSA and 0.01% thimerosal.
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Antigène
T7-Tag
Autre désignation
T7 tag
Classe de substances
Tag
Sujet
Rabbits were immunized with MASMTGGQQMG (T7 epitope) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity-purified using the peptide conjugated to a solid-phase support and then conjugated with horseradish peroxidase (HRP). Specification: The affinity-purified antibodies were analyzed using ELISA and Western blot analyses. ELISA: The anti-T7 diluted 1:25,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated with a different carrier.