L’anticorps anti-BTAF1 Monoclonal Souris est utilisé pour la détection de BTAF1 dans des échantillons de Humain. Il a été validé pour WB, IP, FACS, ICC et DB.
Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Dot Blot (DB)
Clone
BTA3D61
Réactivité croisée
Humain, Souris, Rat (Rattus)
Réactivité croisée (Details)
Other species not studied.
Purification
Protein G affinity chromatography
Immunogène
BTAF1 antibody was raised in mouse using recombinant Btaf1 Rna Polymerase Ii, B-Tfiid Transcription Factor-Associated, 170 Kda (Mot1 Homolog, S. Cerevisiae) (Btaf1)
BTAF1
Reactivité: Humain, Souris, Rat
WB, ChIP, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 0.2-2 µg/mL, IP: 100-500 µg/sample, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Concentration
Lot specific
Buffer
BTAF1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3).
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Stock
4 °C/-20 °C
Stockage commentaire
Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.
Antigène
BTAF1
(TBP-Associated Factor 172 (BTAF1))
Autre désignation
BTAF1
Sujet
Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP, MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B, MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity. Synonyms: Monoclonal BTAF1 antibody, Anti-BTAF1 antibody, B-TFIID transcription factor-associated, MOT1 antibody, TAF172 antibody, KIAA0940 antibody, TAFII170 antibody, TAF(II)170 antibody.