KLH anticorps

N° du produit ABIN93503

L’anticorps anti-KLH Polyclonal Lapin est utilisé pour la détection de KLH dans des échantillons de Keyhole Limpet. Il a été validé pour ELISA, WB et IL.

Aperçu rapide pour KLH anticorps (ABIN93503)

Antigène: KLH (Keyhole Limpet Hemocyanin (KLH))

Reactivité: Keyhole Limpet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KLH est non-conjugé

Application: ELISA, Western Blotting (WB), Immunolocalization (IL)

Détail du produit anti-KLH anticorps

Fonction: Anti-KLH | Keyhole limpet hemocyanin

Réactivité croisée (Details): Megathura crenulata - most commonly used carrier protein
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Purification: Immunogen affinity purified serum

Immunogène: Purified keyhole limpet hemocyanin (KLH)

Isotype: IgG

Information d'application

Indications d'application: 1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL)

Commentaires:

Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Détails sur KLH

Antigène: KLH (Keyhole Limpet Hemocyanin (KLH))

Sujet:

Background: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host's immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

Additional information: Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide.

Poids moléculaire: ca. 400 kDa/subunit

UniProt: Q6KC56