KLH anticorps

N° du produit ABIN93503

Détail du produit anti-KLH anticorps

Antigène: KLH (Keyhole Limpet Hemocyanin (KLH))

Reactivité: Keyhole Limpet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KLH est non-conjugé

Application: ELISA, Western Blotting (WB), Immunolocalization (IL)

Homologie: Megathura crenulata - most commonly used carrier protein

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: Aprox. 400 kDa

Purification: serum

Immunogène: purified keyhole limpet hemocyanin (KLH), whole molecule

Isotype: IgG

Information d'application

Indications d'application: Recommended Dilution: 1 : 10 000 (ELISA), 1 : 10 000 (WB), 1 : 1000 (IL).

Commentaires:

Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution please add 50 µL of dest. water

Buffer: PBS pH 7.4

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Détails sur KLH

Antigène: KLH (Keyhole Limpet Hemocyanin (KLH))

Abstract: KLH Produits

Sujet: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host's immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

Poids moléculaire: ca. 400 kDa/subunit

UniProt: Q6KC56