D1 Protein of PSII, (PsbA) (C-Term) anticorps

N° du produit ABIN93523

Détail du produit

Antigène: D1 Protein of PSII, (PsbA)

Épitope: C-Term

Reactivité: Arabidopsis thaliana, Chlamydomonas reinhardtii, Amphidinium carterae, Chlamydomonas raudensis, Fucus vesiculosus, Gonyaulax polyedra, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Lobaria pulmonaria, Pinus sylvestris, Stony Coral (Turbinaria)

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Specificité: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 ATP synthase and most knownbacterial F-type ATP synthases. Peptide used for antibody product.

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 38 / 28-30 kDa

Purification: affinity purified

Immunogène: KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others

Isotype: IgY

Information d'application

Indications d'application: Recommended Dilution: 1 : 4000 - 1 : 8000 on 5 µg of total protein, detected with standard ECL (WB).
A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cellsprior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands. Anti-PsbA antibodies willnot detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.

Commentaires:

The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions.In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.This antibody will also detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS pH 8.0+ 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Make aliquots to avoid working with a stock.
Please, Remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubek.

Stock: 4 °C

Référence

Muktiono, Schulten, Heemken, Gandrass, Prange, Schnabl, Cerboncini: "Isolation and purification assay of ex vivo photosystem II D1 protein toward integrated biointeraction analysis." dans: Analytical and bioanalytical chemistry, Vol. 390, Issue 4, pp. 1195-202, (2008) (PubMed).

Garczarek, Dufresne, Blot, Cockshutt, Peyrat, Campbell, Joubin, Six: "Function and evolution of the psbA gene family in marine Synechococcus: Synechococcus sp. WH7803 as a case study." dans: The ISME journal, Vol. 2, Issue 9, pp. 937-53, (2008) (PubMed).

Drath, Kloft, Batschauer, Marin, Novak, Forchhammer: "Ammonia triggers photodamage of photosystem II in the cyanobacterium Synechocystis sp. strain PCC 6803." dans: Plant physiology, Vol. 147, Issue 1, pp. 206-15, (2008) (PubMed).

Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." dans: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).

Kuepper, Setlik, Seibert, Prasil, Setlikova, Strittmatter, Levitan, Lohscheider, Adamska, Berman-Frank: "Iron limitation in the marine cyanobacterium Trichodesmium reveals new insights into regulation of photosynthesis and nitrogen fixation." dans: The New phytologist, Vol. 179, Issue 3, pp. 784-98, (2008) (PubMed).

Takahashi, Whitney, Itoh, Maruyama, Badger: "Heat stress causes inhibition of the de novo synthesis of antenna proteins and photobleaching in cultured Symbiodinium." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 11, pp. 4203-8, (2008) (PubMed).

Détail du antigène

Antigène: D1 Protein of PSII, (PsbA)

Sujet: AGI Code: ATCG00020
The PsbA (D1) protein of Photosystem II is rapidly cycled under illumination in all oxygenic photobionts. Disruption of PsbA cycling or losses of PsbA pools are central to photoinhibition of photosynthesis in cyanobacteria, algae and plants under a wide range of conditions including excess light, low temperature and UV exposure. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.

Poids moléculaire: expected: 38 kDa, apparent: 28-30 kDa

UniProt: P14660, P0C434, P83755, Q14FH6