RBCL (Isoform 1) anticorps

N° du produit ABIN93524

Il existe 3+ publications pour ce produit. L’anticorps anti-RBCL Polyclonal Poulet est utilisé pour la détection de RBCL dans des échantillons de Arabidopsis thaliana, Alfalfa, Pea, Synechococcus sp., Spinach, Lobaria pulmonaria, Ulmus, Thiobacillus, Potato, Diatom (Fragilariopsis), Microcystis aeruginosa, Spartina alterniflora et Pinus strobus. Il a été validé pour WB, EM, IL et IF.

Aperçu rapide pour RBCL (Isoform 1) anticorps (ABIN93524)

Antigène: RBCL

Reactivité: Arabidopsis thaliana, Alfalfa, Pea, Synechococcus sp., Spinach, Lobaria pulmonaria, Ulmus, Thiobacillus, Potato, Diatom (Fragilariopsis), Microcystis aeruginosa, Spartina alterniflora, Pinus strobus

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Electron Microscopy (EM), Immunolocalization (IL), Immunofluorescence (IF)

Détail du produit

Épitope: Isoform 1

Fonction: Anti-RbcL | Rubisco large subunit, form I (chicken)

Réactivité croisée (Details): Arabidopsis thaliana, Fragilariopsis cylindrus, Lobaria pulmonaria, Medicago sativa, mixed phytoplankton, Microcystis aeruginosa PCC7806, Pinus strobus, Pisum sativum, Solanum tuberosum, Spartina alterniflora, Spinacia oleracea, Synechococcus sp. PCC7842, Thiobacillus sp. Ulmus sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Algae,Dicots,Conifers,Liverworts,Mosses,Prochlorophytes,Welwitschia

Purification: Purified, total IgY

Immunogène: KLH-conjugated synthetic peptide derived from all known plant

Information d'application

Indications d'application: (IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 μg of total cellular protein, (WB)

Commentaires:

This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 16 µg/µL

Buffer: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: 4 °C

Stockage commentaire: Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Référence

Abdel-Ghany, Pilon: "MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis." dans: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15932-45, (2008) (PubMed).

Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." dans: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).

Menon, Dou, Heinhorst, Shively, Cannon: "Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species." dans: PLoS ONE, Vol. 3, Issue 10, pp. e3570, (2008) (PubMed).

Détail du antigène

Antigène: RBCL

Sujet:

Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.

Additional information: Peptide target used to elicit this antibody is not conserved in type II Rubisco found in dinoflagellates and some photosynthetic bacteria

Poids moléculaire: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)

ID gène: 844754

UniProt: O03042, Q3ALL1