Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Pseudomonas aeruginosa only
Attributs du produit
Expected / apparent Molecular Weight of the Antigene: 48 kDa
amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.
1: 5000 (WB)
For Research Use only
PBS pH 8.0+ 0.02 % sodium azide
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes. Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.
store at 4°C, make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Shen, Tsay, Chia, Wu, Lee, Hu: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." dans: Investigative ophthalmology & visual science, (2012) (PubMed).
(ExoS Gene Product (EXOS))
Exoenzyme S (ExoS)
Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.