EXOS anticorps (cleaved)

N° du produit ABIN93557

Il existe 1 publication pour ce produit. L’anticorps anti-EXOS Polyclonal Poulet est utilisé pour la détection de EXOS dans des échantillons de Pseudomonas aeruginosa. Il a été validé pour WB.

Aperçu rapide pour EXOS anticorps (cleaved) (ABIN93557)

Antigène: EXOS (ExoS Gene Product (EXOS))

Reactivité: Pseudomonas aeruginosa

Hôte: Poulet

Clonalité: Polyclonal

Application: Western Blotting (WB)

Détail du produit anti-EXOS anticorps

Épitope: cleaved

Fonction: Anti-ExoS | Exoenzyme S

Réactivité croisée (Details): Pseudomonas aeruginosa
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Pseudomonas aeruginosa

Purification: Purified, total IgY

Immunogène: amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.

Isotype: IgY

Information d'application

Indications d'application: 1 : 5000 (WB)

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: 4 °C

Stockage commentaire: Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Références pour anticorps anti-EXOS (ABIN93557)

Shen, Tsay, Chia, Wu, Lee, Hu: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." dans: Investigative ophthalmology & visual science, (2012) (PubMed).

Détails sur EXOS

Antigène: EXOS (ExoS Gene Product (EXOS))

Autre désignation: Exoenzyme S (ExoS)

Sujet: Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.

Poids moléculaire: 48 kDa

UniProt: Q51449