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EXOS anticorps (cleaved)

EXOS Reactivité: Pseudomonas aeruginosa WB Hôte: Poulet Polyclonal unconjugated
N° du produit ABIN93557
  • Antigène
    EXOS (ExoS Gene Product (EXOS))
    Épitope
    cleaved
    Reactivité
    Pseudomonas aeruginosa
    Hôte
    Poulet
    Clonalité
    Polyclonal
    Application
    Western Blotting (WB)
    Réactivité croisée (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    Homologie
    Pseudomonas aeruginosa only
    Attributs du produit
    Expected / apparent Molecular Weight of the Antigene: 48 kDa
    Purification
    affinity purified
    Immunogène
    amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.
    Isotype
    IgY
  • Indications d'application
    1: 5000 (WB)
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS pH 8.0+ 0.02 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
    Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.
    Stock
    4 °C
    Stockage commentaire
    store at 4°C, make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
  • Shen, Tsay, Chia, Wu, Lee, Hu: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." dans: Investigative ophthalmology & visual science, (2012) (PubMed).

  • Antigène
    EXOS (ExoS Gene Product (EXOS))
    Autre désignation
    Exoenzyme S (ExoS)
    Sujet
    Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.
    Poids moléculaire
    48 kDa
    UniProt
    Q51449
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