PsbO anticorps

N° du produit ABIN93573

Détail du produit

Antigène: PsbO

Reactivité: Arabidopsis thaliana, Orge, Nicotiana tabacum

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Réactivité croisée (Details): No cross-reactivity with: Chlamydomonas reinhardtii, Synechococcus

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 33 kDa

Purification: serum

Immunogène: N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 At5g66570 and 2 proteins At5g50820

Information d'application

Indications d'application: Recommended Dilution: 1 : 1000 with standard ECL (WB).
Loading based on 50 - 100 ng of chlorophyll is enough to obtain good signal withthis antibody

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL, of sterile water.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Référence

Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." dans: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

Lundin, Nurmi, Rojas-Stuetz, Aro, Adamska, Spetea: "Towards understanding the functional difference between the two PsbO isoforms in Arabidopsis thaliana--insights from phenotypic analyses of psbo knockout mutants." dans: Photosynthesis research, Vol. 98, Issue 1-3, pp. 405-14, (2008) (PubMed).

Détail du antigène

Antigène: PsbO

Sujet: AGI Code: At5g66570
The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.

Poids moléculaire: 33 kDa

UniProt: Q9S841, P23321