TUBB anticorps

N° du produit ABIN93915

Cet anticorps anti-TUBB est un anticorps Souris Monoclonal détectant TUBB dans WB, ICC et ICFC. Adapté pour Humain, Souris, Rat, Porc, Nicotiana tabacum et Arabidopsis. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour TUBB anticorps (ABIN93915)

Antigène: TUBB (Tubulin, beta (TUBB))

Reactivité: Humain, Souris, Rat, Porc, Nicotiana tabacum, Arabidopsis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBB est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Clone: TU-12

Détail du produit anti-TUBB anticorps

Fonction: Anti-beta-Tubulin Purified

Specificité: The antibody TU-12 recognizes an epitope located within aa 345-430 of C-terminal domain of beta-tubulin in various species.

Réactivité croisée (Details): Broad species reactivity

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Porcine brain microtubule protein MTP-1.

Isotype: IgM

Information d'application

Indications d'application: Immunocytochemistry: Recommended dilution: 2-5 μg/mL, fixed and permeabilized cells.
Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.
Flow cytometry: Recommended dilution: 4-10 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-TUBB (ABIN93915)

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." dans: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, (1997) (PubMed).

Détails sur TUBB

Antigène: TUBB (Tubulin, beta (TUBB))

Autre désignation: beta-Tubulin

Sujet: Tubulin beta,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.,TUBB

ID gène: 81027

UniProt: Q9H4B7

Pathways: Dynamique des Microtubules, M Phase