Blood Group Lewis A anticorps

N° du produit ABIN93924

Détail du produit

Antigène: Blood Group Lewis A

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA

Specificité: The mouse monoclonal antibody 7LE recognizes Lewis^a blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed on colonic epithelial cells. Lewis^a may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.

Réactivité croisée (Details): Other not tested, Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).

Clone: 7LE

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive control: colorectal adenocarcinoma, heat mediated antigen retrieval (sodium citrate), mAb incubation 1 hour / RT, detection DAB.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Creuzot-Garcher, Guerzider, Assem, Bron, Delannoy, Bara: "Alteration of sialyl Lewis epitope expression in pterygium." dans: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, (1999) (PubMed).

"First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems." dans: Revue française de transfusion et immuno-hématologie, Vol. 31, Issue 2, pp. 115-486, (1989) (PubMed).

Détail du antigène

Antigène: Blood Group Lewis A

Synonymes: anticorps CD174, anticorps FT3B, anticorps FucT-III, anticorps LE, anticorps Les, anticorps fucosyltransferase 3 (Lewis blood group), anticorps FUT3