ACRV1 anticorps

N° du produit ABIN93928

Cet anticorps Souris Monoclonal détecte spécifiquement ACRV1 dans WB et ICC. Il présente une réactivité envers Chien et a été mentionné dans 1 publication.

Aperçu rapide pour ACRV1 anticorps (ABIN93928)

Antigène: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Reactivité: Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ACRV1 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: Ds-2

Détail du produit anti-ACRV1 anticorps

Fonction: Anti-Can Intra-Acrosomal Protein Purified

Specificité: The mouse monoclonal antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.

Réactivité croisée (Details): Canine (Dog)

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.

Isotype: IgG

Information d'application

Indications d'application: Immunocytochemistry: Membrane permeabilization (acetone) is essential.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-ACRV1 (ABIN93928)

Geussová, P?knicová, Capková, Kaláb, Moos, Philimonenko, Hozák: "Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction." dans: Andrologia, Vol. 29, Issue 5, pp. 261-8, (1997) (PubMed).

Détails sur ACRV1

Antigène: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Autre désignation: Intra-Acrosomal Protein

Sujet: One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,IAP Can